The use of computer-assisted experiments in the canine to support the development of a mathematical model for the hepatic removal of indocyanine green dye under normal and reduced hepatic blood flow conditions

摘要

Sixteen mongrel dogs were tested for hepatic removal of indocyanine green (ICG) dye in normal animals and animals with portal-caval shunts. Characteristics of blood ICG clearance and bile ICG accumulation were studied. Response of the mathematical model is similar to the response from the studies of normal animals when programmed with initial parameters taken from the animal studies. A data-handling system was developed for the acquisition of the experimental data. Cardiac output decreased two-fold (p \u3c 0.01) following a portal-caval shunt. Dye decay rate in the plasma decreased (p \u3c 0.05) which resulted in an increase in area under the blood concentration curve and time to zero concentration in the shunted animals. Mean bile flow was reduced two-fold (p \u3c 0.05) while the area under the ascending bile curve increased (p \u3c 0.05) in the shunted animals. A method in which to measure the volume of the bile canalicular space has been discovered by analysis of the descending portion of the bile dye curve. Effects of methoxyfluorane-nitrous oxide anesthetic on the hepatic system appear to be minimal with respect to dye clearance as seen by relatively small differences between the animal data and the mathematical model.